APPLICATION OF MOLECULAR TOOLS IN ANIMAL BREEDING, CROP SCIENCE, FOOD CONTROL AND AGROBIODIVERSITY IN THE REPUBLIC OF MACEDONIA

Zoran T. Popovski, Blagica Tanaskovska, Elizabeta Miskoska – Milevska, Tome Nestorovski, Koco Porcu, Katerina Bandzo-Oreshkovikj, Milica Svetozarevic, Zimera Saiti, Macdonald Wick

Abstract


Molecular methods have become non-replaceable for the improvement of food production and its control. The Department for Biochemistry and Genetic Engineering (DBGE) as a part of the Faculty of Agriculture Sciences and Food is a crucial entity using molecular techniques in different areas of agriculture and livestock production. DBGE is mainly dealing with marker assisted selection (MAS), evolutionary studies; determine the origin of food products; enzyme analysis in food processing; transcriptional and translational analyses; molecular detection of plant viruses; and GMO analysis. The target molecules were proteins, RNA and DNA. MAS is systematically applied in animal breeding in Macedonia using RYR-1, k and αS1-casein genes. Evolutionary studies were performed using DNA microsatellites to estimate genetic distance among autochthonic strains of sheep and tomato varieties. The identification of food products origin and gender identification in fish was done using protein profiling. Gene expression was studied analysing different growth factors and inhibitors at RNA level. More than 3.000 plant and animal samples were analysed in the Laboratory for biochemistry and molecular biology. GMO laboratory as a part of the DBGE is the first authorized facility for GMO testing of food and feed samples. In more than 100 samples RT PCR methods were used.

Keywords


livestock; crops; food; molecular tools; Macedonia

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References


Current applications of biotechnology, Editor in Chief Munis Dündar, Fabrizio Bruschi, Kevan Gartland, Mariapia, Viola Magni, Peter Gahan, Yusuf Deeni, European Biotechnology Thematic Network Association, 2013.

J. L. Williams: The use of marker-assisted selection in animal breeding and biotechnology Rev. sci. tech. Off. int. Epiz., 24 (1) (2005), pp. 379–391.

J. Franco, J. Crossa, J. M. Ribaut, J. Betran, M. L. Warburton, M. Khairallah, A method forcombining molecular markers and phenotypicattributes for classifying plant genotypes, Theor. Appl. Genet, 103 (6–7) (2001), pp. 944–952.

J. C. M. Dekkers: Commercial application of marker and gene assisted selection in livestock: strategies and lessons, J. Anim. Sci., 82 (2004), pp. 313–328.

R. Djedović, G. Trifunović, D. Stanojević: Ge-nomic Selection. Proceedings of the First Interna-tional Symposium on Animal Science. Belgrade, Serbia. Book I, (2012), pp. 207–216.

C. He, V. Poysa, K.Yu, Development and charac-terization of simple sequence repeat (SSR) markers and their use in determining relationship among Ly-copersicum esculentum cultivars, Theor. Appl. Genet., 10 (2003), pp. 363–373.

Y. Jiao, H. Jia, X. Li, M. Chai, H. Jia, Development of simple sequence repeat (SSR) markers from a ge-nome survey of Chinese bayberry (Myricarubra), BMC Genomics, 13 (2012), pp.1–16.

L. Herrera-Estrella, A. Depicker, M. van Montagu, J. Schell, Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector, Nature 303 (1983), pp. 209–213.

J. Zel, M. Mazzara, C. Savini, S. Cordeil, M. Cam-loh, D. Štebih, K. Cankar, K. Gruden, D. Morisset, G. Van den Eede, Method Validation and Quality Management in the Flexible Scope of Accredita-tion: An Example of Laboratories Testing for Ge-netically Modified Organisms, Food Anal. Meth., (1), Issue 2, (2008), pp. 61–72.

W. Dong, L. Yang, K. Shen, B. Kim, G. A. Kleter, J. P. Marvin, H. R. Guo, W. Liang, D. Zhang, GMDD: a database of GMO detection methods, BMC Bioinformatics, 9 (2008): 260.

P. Zachar. Identification of milk and milk products, Mljekarstvo 61 (3) (2011), pp. 199–207.

K. Mullis, F. Faloona, S. Scharf, R. Saiki, G. Horn, H. Erlich: Specific enzymatic amplification of DNA in vitro. Polymerase chain reaction. Cold Spring Harbor, Symp. Quant. Biology (1986), pp. 51–263.

J. Fujii, K. Otsu, F. Zorzato, S. de Leon, V. K. Khanna, J. E. Weiler, P. J. O'Brien, D. H. MacLen-nan: Identification of a mutation in Porcine Ryanodine Receptor Associated with Malignant Hyperthermia, Science, 253 (1991), pp. 448–451.

K. Otsu, V. K. Khanna, A. L. Archibald, D. H. MacLennan: Cosegregation of porcine malignanthyperthermia and a probable causal mutation in the sceletal muscle ryanodine receptor gene in back-cross families, Genomics, 11 (3) (1991), pp.744–750.

Z. T. Popovski, B. Tanaskovska, K. Porchu, V. Vukovic, S.Andonov, B.Palasevski: New Approach in Detection of Porcine Stress Syndrome, Biotech-nolog in Animal Husbandry, 18 (5–6) (2002), pp. 73–80.

Z. T. Popovski, B. Dimitrievska, S. Andonov, V. Vukovic, S. Domazetovska: Corelation between certain biochemical parameters and different geno-types for malignant hyperthermia in swine. First in-ternational sympoosium on animal science Bel-grade, Serbia. Proceedings – Book of papers (2012) pp. 258–264.

Z. T. Popovski, Z. Pejkovski, T. Nestorovski: Stress free populations deliver quality. Fleisch Wirtschaft International, 31 – 5, (2016), pp.17–21.

E. Jakob, Genetic Polymorphism of milk proteins, Mljekarstvo, 44 (3) (1994), pp. 197–217.

Z. T. Popovski, B. Palasevski, N. Dubrova-Mateva, V. Andonovska, Z. Naletovski, Polymorphisms of --casein and lactoglobulin in cow milk

Sympo-sia for Livestock production, Year Book (2001) pp. 45–50.

A. Barroso, S. Dunner, J. Canon, Detection of Bo-vine Kappa Casein Variants A, B, C and E by Means of Polymerase Chain Reaction – Single Strand Conformation Polymorphism (PCR-SSCP), J. Anim. Sci., 76 (1998), pp. 1535–1538.

J. C. Mercier, G. Brigman, D. Ribadeau, Primary structure of bovine -kasein B. Complete se-quence, Eur. J. Biochem., 35 (1973), pp. 222–235.

D. Morini, G. Losi, G. B. Castagnetti, P. Mariani, Cheesemaking experiments with milk characterized by kappa casein variants A and B: characteristics of the ripened cheese, Scien. Tecn. Latt.-Casear, 30 (1979), pp. 243–262.

B. Tanaskovska (Dimitrievska), K. Porcu, Z. T. Popovski, Frequency of -casein Genotypes in Hol-stein-Frisian Cattle in Republic of Macedonia, Bio-technology, Scientific Pedagogical Publishing, Ceske Budejovice, Czech Republic, (2006) ISBN 8085645-53-X pp. 415–417.

H. E. Swaisgood, Chemistry of the caseins. In: Fox PF, editor. Advanced Dairy Chemistry. London: Elsevier, 1992, pp. 63–110.

F. Pilla, C. Bevilacqua, C. Leroux, A. Fraghi, P. Martin: Genotiping of alpha s1 casein in sheep. Anim. Genet., 29 (1998), pp. 460–477.

Z. T. Popovski, B. Dimitrievska, K. Porcu, M. Brka, A. Rustempasic, B. Markovic, R. Djedovic, V. Mar-geta, H. Mehmeti, M. Vegara: Polymorphisms in ovines1-casein gene among autochthon strains of pramenka breed sheep in Balkan. International symposium for Agriculture and food (2013) Sym-posium proceedings pp. 687–690.

B. Vaskov, G. Efremov, Fourth Haemoglobin Type in Sheep, Nature, 216 (1967), pp. 593–594.

Z. T. Popovski, V. Dzabirski, S. Andonov, S. Tro-jacanec, Polymorphisms of some serum proteins and hemoglobin in conserved nucleus of Kara-kachan sheep in Macedonia, Symposia for Live-stock production, Year Book, 2001, pp.87–91.

G. Kahl, The Dictionary of Gene Technology. Wiley-WCH, Winheim (2001).

K. Belkhir, P. Borsa, J. Goudet, L. Chikhi, F. Bon-homme, GENETIX, Montpellier, University of Montpelier, (1988).

M. Cinkulov, Z. Popovski, K. Porcu, B. Ta-naskovska, A. Hodzic, H. Bytyqi, H. Mehmeti, V. Margeta, R. Djedovic, A. Hoda, R. Trailovic, M. Brka, B. Markovic, B. Vazic, M. Vegara, I. Olsaker, J. Kantanen, Genetic diversity and structure of the West Balkan Pramenka sheep types as revealed by microsatellite and mitochondrial DNA analysis, J. Anim. Breed. Genet., 125 (2008), pp. 417–426.

Min Du, R. J. McCormick, Taylor & Francis Group, Applied muscle biology and meat science USA, (2009).

T. Laas, In protein purification: principles, high res-olution methods and applications (ed. J.-C. Janson and L. Ryden), VCH press, Weinheim (1989).

J. C. Sawdy, S. A. Kaiser, N. R. St-Pierre, M. P. Wick, Myofibrillar 1-D fingerprints and myosin heavy chain MS analyses of beef loin at 36 h post-mortem correlate with tenderness at 7 days, Meat Science, 67 (2004), pp. 421–426.

T. Nestorovski, B. Tanaskovska, M. Wick, M. Svetozarevic, Z. T. Popovski, The influence of size and living temperatures on the muscle protein pro-file of koi carp (Cyprinus carpio haematopterus). II International Symposium for Agriculture and Food. Book of abstracts, Ohrid (2015). pp. 355.

L. M. Z. Chicharo, M. A. A. T Chicharo, The DNA/RNA ratio as a useful indicator of the nutri-tional condition in juveniles of Ruditapes decus-satus. Sarda, R., Ros, J.D. (Eds.), Scientia Marina, (1995) pp. 95–101.

Z. T. Popovski, K. Kwasek, M. Wojno, K. Dabrowski, B. Tanaskovska, S. Andonov, M. Wick: Determination of RNA DNA ration in white muscle samples of koi carp using different techniques. II International Symposium for Agriculture and Food. October 2015, Ohrid. Book of abstracts p.349.

S. Surinova, R. Schiess, R. Huttenhain, F. Cerciello, B. Wollscheid, R. Aebersold, On the Development of Plasma Protein Biomarkers, J. Proteome Res., 10 (2011), pp. 5–16[39] Z. T. Popovski, B. R. Dimitrievska, E. Miskoska – Milevska, K. Bandzo: Electrophoretic determina-tion of female specific protein in koi carp as a tool for gender identification: Technique comparison. International symposium for Agriculture and food, Symposium proceedings (2013) pp.691–695.

G. Mennella., V. Onofaro Sanaja, A. Tonnini, V. Magnifico, Seed storage protein characterization of Solanum species and of cultivars and androgenetic lines of S. melanogena L. by SDS-PAGE and AE-HPLC, Seed Sci. Technol., 27 (1999), pp.23–35.

A. Hussain, Interlines variation of electrophore-grams of cotyledon protein of field bean, Euphitica, 39 (1988), pp.109–113.

E. Miskoska-Milevska, Z. T. Popovski, B. Dimi-trievska, K. Porcu, Differences in Tomato Seed Pro-tein Profiles Obtained by SDS PAGE Analysis, J. Agricul. Sci., 53 (2008), pp. 13–23.

A. E. Alvarez, C. C. M. Van De Wiel, M. J. M. Smulders, B. Vosman, Use of microsatellites to evaluate genetic diversity and species relationships in the genus Lycopersicon, Theor. Appl. Genet., 103 (2001), pp. 1283–1292.

E. Miskoska – Milevska, Z. T. Popovski, Blagica R. Dimitrievska, K. D. Porcu, Isolation of DNA for fragment analyses from tomato leaves and seeds. XVI International Conference of Biotechnology with international participation, Faculty of Agron-omy, Čačak, 2011, pp. 59–64.

E. Miskoska – Milevska, Z. T. Popovski, T. Nes-torovski, Usefulness of a locus LE21085 in the ge-netic differentiation of tomato varieties, Genetika, 9 (2) (2017), pp. 2019–2023.

M. Slatkin, A measure of population subdivision based on microsatellite allele frequencies, Genetics, 139 (1) (1995), pp. 457–462.

E. Miskoska – Milevska, Z. T. Popovski, Blagica R. Dimitrievska, K. D. Porcu., K. Bandzo, Determina-tion of genetic diversity among different tomato va-rieties using SSR markers, Acta Agriculturae Ser-bica, Vol. XVI, 31 (2011), pp. 9–17.

G. Parrella, N. Acanfora, M. G. Bellardi, First Rec-ord and Complete Nucleotide Sequence of Alfalfa mosaic virus from Lavandula stoechas in Italy. Plant Disease – Disease Notes, 94 (7) (2010), p.924.

K. Bandzo, R. Rusevski, B. Kuzmanovska, S. Ban-dzo, E. Miskoska-MIlevska, Z. T. Popovski, Sero-logical approach in the detection of viruses in pep-per plants cultivated on open fields in the Republic of Macedonia. International scientific conference “Challenges in modern agricultural production” Book of

abstracts, 2014, p. 62.

B. (Dimitrievska) Tanaskovska, K. Porcu, Z.T. Popovski: Development of a System for GMO Test-ing of Food, Seed and Feed Samples in the Republic of Macedonia, XI Savetovanje o Biotehnologiji, 2006, pp. 665–669.

G. J. van Duijn, R. van Biert, H. Bleeker-Marcelis, I. Van Boeijen, A.J. Adan, S. Jhakrie, M. Hessing Detection of genetically modified organisms in foods by protein and DNA-based techniques: bridg-ing the methods. J AOAC Int. 85(3) (2002): pp. 787–91

З. Поповски, Э. Мискоска-Милевска, Т. Несторовски, Е. В. Камалдинов, В. Л. Петухов: Подходы к обнаружению генно-модифицированной кукурузы и проведению полуколичественного анализа с использованием белка СP4 EPSPS. (Approaches to investigation of gene modified maize and semi-quantative analysis by means of СР4 EPSPS protein). Вестник НГАУ, 3 (40) (2016), pp. 92–97.

B. Dimitrievska, Z. T. Popovski, K. Porcu: Merging procedures for detection of soy DNA and presence of GM soy in food samples. Foodinova 2010, Va-lencia, Spain (2010), pp.146

M. Svetozarevic, T. Nestorovski, Z. T. Popovski, Electrophoretic distinction of the origin in different dairy products and milk samples. International sym-posium on animal science, Beograd, Serbia, Sep-tember 23-25, Proceedings of papers. (2014). pp. 551–557.

I. Irfan, A. Arslan, Identification of Meat Species by Polymerase Chain Reaction Technique, Turkish Journal of Veterinary and Animal Science, 31 (3) (2007), pp. 159–163.

Z. S. Musliji, M. Svetozarevic, T. Nestorovski, B. R. Tanaskovska, Z. T. Popovski, Different ap-proaches in the identification of meat origin based on protein profiling and simple PCR. II Interna-tional Symposium for Agriculture and Food (2015) Ohrid. Book of abstracts, pp. 142.

J. S. Fruton, A history of pepsin and related en-zymes, Q. Rev. Biol., 77 (2) (2002), pp. 127–47

T. Nestorovski: Determination of purity and con-centration of chymosin and its equivalents in com-mercial samples. Master work (2017) 43 pp.




DOI: http://dx.doi.org/10.20903/csnmbs.masa.2017.38.2.112

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