HISTOCHEMICAL EVALUATION OF IRON CONTENT IN THE LIVER OF WILD FEMALE OHRID TROUT (SALMO LETNICA KAR.) ALONG THE BREEDING CYCLE

Maja Jordanova Jordanova, Katerina Rebok, Maria João Rocha, Eduardo Rocha

Abstract


Iron is an essential micronutrient for the normal metabolism of all cells. Intracellular iron varies within normality ranges, and depletion or overload of brings serious consequences. As such, iron can also be deleterious when appearing as an environmental toxicant. The aim of this study was to estimate semi-quantitatively the liver iron content in female Ohrid trout, from the Lake Ohrid, during the breeding cycle. Seasonal variations in hepatocytic iron were observed. The maximum content occurred at post-spawning, which was significantly higher than loads detected at pre- and early-vitellogenesis. Positive correlations existed between iron amounts and stages of ovary maturation. By the contrary, there were no correlations between iron contents and plasma oestradiol levels. The new uncovered "iron-breeding associa-tion" strongly endorses that normal seasonal variations in hepatocytic iron content should be taken into consideration in field studies investigating and monitoring the metal burden and toxicological effects of metals on wild fish.

Keywords


iron; liver; Ohrid trout; oestradiol; seasonal

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DOI: http://dx.doi.org/10.20903/csnmbs.masa.2018.39.2.125

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