CHARACTERIZATION OF A LOCUS LECH13 IN DIFFERENT TOMATO VARIETIES USING FRAGMENT ANALYSES

Elizabeta Miskoska Milevska, Zoran T. Popovski, Tome Nestorovski

Abstract


The microsatellite markers are routinely used to investigate the genetic structure of natural populations. The microsatellite
polymorphisms are important for estimation diversity among varieties and for evaluation of the efficiency of
microsatellite for establishing varieties relationships. The locus LECH13 was tested in six tomato varieties in order to
evaluate its usefulness in the genetic differentiation among six morphologically different tomato varieties. The fragment
analyses were performed using the Applied Biosystems DNA analyzer. The number of detected alleles for the microsatellites
locus LECH13 was four in researched tomato varieties (134-136-138-146 bp). The allele138 bp was noticed only
in Lycopersicon esculentum subsp. spontaneumvar. racemigerum. The average PIC value for the locus LECH13 was
0.3677 and it belongs to the group of modest informative markers. The present study showed that the locus LECH13
could be used in the genetic differentiation of tomato varieties, but in combination with other polymorphic microsatellite
loci.


Keywords


DNA microsatellites, fragment analyses, locus LECH13, tomato, varieties

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DOI: http://dx.doi.org/10.20903/csnmbs.masa.2019.40.2.151

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